Rich in flavonoids, Propolis consistenly exhibits strong antioxidant, anti-inflammatory, antiviral, antifungal, anti-tumoral activity. This study effectively identifies the active flavonoids and phenolic acids which produced these positive results...
Chemical
Composition, Cytotoxic and Antioxidative Activities of Ethanolic Extracts of
propolis on HCT-116 Cell Line
Journal of the Science of Food and Agriculture, 2013, Accepted Article
Background:
Propolis is
a complex resinous sticky substance that honeybees collect from buds and
exudates of various plants. Due to propolis versatile biological and
pharmacological activities, it is widely used in medicine, cosmetics and food
industry. The aim of this study was to evaluate cytotoxic and antioxidative
effects of various ethanolic extracts of propolis (EEP) on human colon cancer
cell line (HCT-116) and compare it with their composition. HPLC-DAD method was
used to determine the chemical composition of propolis samples.
Results:
The most
abundant flavonoids in all samples were chrysin, pinocembrin and galangin
(12.697- 40.811 µg mg-1). On the other hand, main phenolic acids were caffeic,
ferulic and isoferulic acid. Dose and time-dependent inhibition of cell growth
of HCT-116 cells was observed in all propolis samples, with IC50 values ranging
from 26.33 to 143.09 µg mL-1. Differences in cytotoxic activity of propolis
samples were associated with differences in their composition. Our results
showed that all EEP samples reduced both superoxide anion radical and nitrite
levels and also had strong DPPH scavenging activity.
Conclusion:
All tested
propolis samples had pronounced cytotoxic and antioxidative activities.
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