Wednesday, August 28, 2013

Honey Protects Liver from Melamine Toxicity

Great news: Propolis has also been found to detoxify the liver. Taking both honey and propolis together also creates a synergistic effect, whereby the benefits of each are enhanced when taken together. Imagine if industrial workers were given a simple dose of honey & propolis before going to work...

Bees' Honey Protects the Liver of Male Rats against Melamine Toxicity
BioMed Research Intl, 2013, June 22

The protective effect of natural bees' honey to the liver of male albino rats against melamine toxicity was studied.

Melamine supplementation at a dose of 20000 ppm in the diet for 28 days induced adverse effects on the liver, decreased serum total protein and increased liver enzyme: alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase. Histological changes of the melamine supplemented group showed necrosis in the hepatic tissues around the central veins of the liver and precipitation of melamine crystals. Treating the male albino rats (that were presupplemented regularly with 20000 ppm melamine) with natural bees' honey at a dose of 2.5 g/kg body weight for 28 days improved both liver functions and increased serum protein.
In addition, a positive impact on the shape of the cells after treatment with honey compared to the positive melamine supplemented group was observed. 

In conclusion, the results of this study revealed that the use of natural bees' honey has the ability to protect the liver of rats against the toxic effects of melamine.

Saturday, August 24, 2013

Bee Venom Aids Wound Healing, Cell Regeneration

This study finds Bee Venom has the potential to advance wound healing practices and we find yet another medicinal use for honey bee products. From these results, it appears one could combine bee venom, propolis and honey to create a super wound healing agent... 

Effects of honeybee (Apis mellifera) venom on keratinocyte migration in vitro
Pharmacogn Mag, 2013, July 9

Since the ancient times the skin aging application of honeybee venom (BV) is practiced and persisted until nowadays. The present study evaluated the effect of the honeybee venom (BV) on keratinocyte migration in wound healing model in vitro.

To access BV further as a cosmetic ingredient and a potential external application for topical uses, we performed studies to investigate the biologic effect of BV treatment on keratinocyte proliferation and migration in vitro.

BV cytotoxicity was assessed by using a 3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl tetrazolium bromide (MTT) assay over 24 h. To assess BV genotoxicity, damage to human epidermal keratinocyte (HEK) was evaluated using the Comet assay. HEK migration was evaluated using a commercial wound healing kit. The skin pro-inflammatory cytokines interleukin (IL)-8 and tumor necrosis factor (TNF)-α were examined to evaluate the pro-inflammatory response to BV.

BV effect on keratinocyte migration
It was found that BV [< 100 ug/ml] was not cytotoxic and stimulated more HEK proliferation and migration compared to negative control, and did not induce DNA damage. There were also decreases in IL-8 and TNF-α expression levels in HEK at all time points.


These findings highlight the potential of topical application of BV for promoting cell regeneration and wound treatment.

Saturday, August 17, 2013

Royal Jelly Improves Sperm Kinematics

Studies of Royal Jelly continue to reveal existence of its protective properties, as well as its consistent ability to enhance sexual function, from women to men and even rams...

Royal Jelly improves the sperm parameters of ram semen during liquid storage and serves as an antioxidant source
SmallRuminant Research  2013, July

The current study was carried out to investigate the protective effects of the Royal Jelly (RJ) supplementation on the sperm kinematics, plasma membrane functionality, the level of produced nitric oxide (NO) and total antioxidant capacity during the liquid storage of ram semen at 4 °C, for various periods of time (0, 24, 48, 72, 96 and 120 h).

Semen samples were collected from four rams and pooled, diluted with Tris–egg yolk extender without RJ (control) or supplemented with different concentrations of RJ (0, 0.5, 1, 1.5 and 2%), at a final concentration of 200 × 106 sperm/mL. Sperm viability, kinematics and membrane functionality were determined by nigrosin–eosin staining, computer-assisted sperm analysis (CASA), and by using the hypo-osmotic swelling test (HOST), respectively. Additionally, the oxidative and nitrosative status were evaluated after the RJ supplementation. The RJ supplementation resulted in a significant (P < 0.05) increase of sperm viability with the highest increase at 1% RJ concentration for 120 h. A significant protective effect of RJ on sperm membrane functionality was obtained at lower concentrations (0.5 and 1%) and in all incubated time points. The most prominent protective effect of RJ on sperm motility parameters was found on the progressive velocity (VSL) as after 72 h storage, no significant reduction was found in comparison to the 50% reduction in the control group. The produced NO level during storage time was reduced by addition of RJ at low concentrations (0.5 and 1%).

Our data suggest that the RJ supplementation at lower concentrations (0.5 and 1%) improves the ram sperm kinetics and plasma membrane functionality during the liquid and cold storage. Moreover, the protective effect of RJ might be attributed to its antioxidative/antinitrosative capacities.

Wednesday, August 7, 2013

Jujube Honey Effective Against Candida

Manuka is not the only plant that provides therapeutic benefits. Other plants, such as Jujube, are reported to have numerous therapeutic properties, listed in Wikipedia. So, it's not surprising to see some of these qualities transformed by honeybees as they turn nectar into healthy, monofloral honey... 

Effect of Jujube Honey on Candida albicans Growth and Biofilm Formation
Arch MedRes, 2013 Jul 15

Candida species, especially Candida albicans, are major fungal pathogens of humans that are capable of causing superficial mucosal infections and systemic infections in humans. The aim of this study was to evaluate the jujube (Zizyphus spina-christi) honey for its in vitro inhibitory activity against pre-formed biofilm and its interference with the biofilm formation of C. albicans.

The XTT reduction assay, scanning electron microscopy (SEM) and atomic force microscopy (AFM) were employed to determine the inhibitory effect of Jujube honey on C. albicans biofilm. Changes in the infrared spectrum after treatment with honey were also determined by Fourier transform infrared (FTIR) spectroscopy.

Jujube honey affects biofilms by decreasing the size of mature biofilms and by disruption of their structure. At a concentration of 40% w/v, it interferes with formation of C. albicans biofilms and disrupts established biofilms. The SEM and AFM results indicated that this type of honey affected the cellular morphology of C. albicans and decreased biofilm thickness.

The present findings show that jujube honey has antifungal properties against C. albicans and has the ability to inhibit the formation of C. albicans biofilms and disrupt established biofilms.

Sunday, August 4, 2013

Honey Protects Testes from Noise Stress

This study reaffirms a consistent trait with many of the honey bee products, in that they protect all species, including humans from negative effects, whether it be noise, stress, vaccine side effects, bacteria, inflammation, chemo & radiation exposure, burns, oxidation, etc. The short answer is taking bee products protects your health and simply adding honey, royal jelly and bee pollen are essentiel in today's world...

The effects of honey and vitamin E administration on apoptosis in testes of rat exposed to noise stress

A variety of stress factors are known to inhibit male reproductive functions. So this study was conducted in order to investigate the effects of honey and vitamin E on the germinative and somatic cells of testes of rats exposed to noise stress. 

Materials and Methods
Mature male wistar rats (n0 = 24) were randomly grouped as follows: Group 1 (honey + noise stress), 2 (vitamin E + noise stress), 3 (noise stress,) and 4 as the control group. In groups 1, 2, and 3, rats were exposed to noise stress. In groups 1 and 2, rats also were given honey and vitamin E, respectively, orally for 50 days. After that, the germinative and somatic cells of testes parenchyma were isolated by digesting the whole testes by a standard method. Next, viability, apoptosis, and necrosis of the cells were evaluated by TUNEL kit and flow cytometry. 

The rates of apoptosis and necrosis of the testicular cells were increased (P = 0.003 and P = 0.001, respectively), but viability of these cells decreased in testes of rats exposed to noise stress (P = 0.003). However, administration of honey and vitamin E were significantly helpful in keeping the cells of testis parenchyma alive, which suffers from noise pollution (P < 0.05 and P < 0.05, respectively). 

Noise stress has negative influences on the cells of testicular tissue by increasing apoptotic and necrotic cells. However, the associated enhancement in healthy cells suggests that honey and vitamin E have positive influences on the testis parenchyma.