Tuesday, September 26, 2017

Buckwheat Honey Exhibits Super Protective Effect

Generally speaking, the darker the honey the richer it is both in mineral content and therapeutic effect. Buckwheat honey has a history of it's beneficial value and this study confirms its high antioxidant activity and phenolic content. Though it may be strong in flavor for the palette, it's an ideal complement to a wound healing strategy.

The Protective Effect of Whole Honey and Phenolic Extract on Oxidative DNA Damage in Mice Lymphocytes Using Comet Assay


In this study, the antioxidant activity and the protective effect against hydrogen peroxide-induced DNA damage were assessed for five honeys of different botanical origin. Seven phenolic acids were detected in the honey samples. Ferulic acid was the most abundant phenolic acid detected in longan honey, jujube honey and buckwheat honey. Ellagic acid, p-hydroxybenzoic acid and protocatechuic acid were the main phenolic acids detected in vitex honey.

Of all honey samples tested, the highest total phenolic content and antioxidant activity were found in buckwheat honey, whereas the lowest total phenolic content and antioxidant activity were found in locust honey. Treatment with hydrogen peroxide induced a 62% increase in tail DNA in mice lymphocytes, and all studied honeys significantly inhibited this effect (P < 0.05).

The buckwheat honey with higher antioxidant capability also exhibited super protective effect than others. Phenolic extracts of honey displayed greater protective effects than whole honey in comet assay. The hydrogen peroxide-generated increase in 8-hydroxy-2-deoxyguanosine (8-OHdG) was effectively inhibited by the honeys studied (P < 0.05). Moreover, a dose-effect relationship between honey concentration and its protective effect was clearly observed in this study.


It can be deduced that phenolic acids of honey can penetrate into lymphocytes and protect DNA from oxidative damage by scavenging hydrogen peroxide and/or chelating ferrous ions.


Friday, September 30, 2016

Antimicrobial Effects of Royal Jelly Confirmed

Deserving of its name, Royal Jelly is indeed very remarkable! Not only does it provide hormonal support in men and women, acts as a "brain booster" aiding mental capacities, it also has significant antimicrobial force, especially against Gram positive bacteria. 

Royal Jelly: An Ancient Remedy with Remarkable Antibacterial Properties
Microbiol Res. 2016 Nov


Abstract
Royal Jelly (RJ), a honeybee hypopharyngeal gland secretion of young nurse and an exclusive nourishment for bee queen, has been used since ancient times for care and human health and it is still very important in traditional and folkloristic medicine, especially in Asia within the apitherapy.

Recently, RJ and its protein and lipid components have been subjected to several investigations on their antimicrobial activity due to extensive traditional uses and for a future application in medicine. Antimicrobial activities of crude Royal Jelly, Royalisin, 10-hydroxy-2-decenoic acid, Jelleines,

Major Royal Jelly Proteins against different bacteria have been reported. All these beehive products showed antimicrobial activities that lead their potential employment in several fields as natural additives. RJ and its derived compounds show a highest activity especially against Gram positive bacteria. The purpose of this Review is to summarize the results of antimicrobial studies of Royal Jelly following the timescale of the researches.

From the first scientific applications to the isolation of the single components in order to better understand its application in the past years and propose an employment in future studies as a natural antimicrobial agent.


Sunday, September 18, 2016

Honey is for Horses and Humans, Heals Wounds

The take-away from this study is that honey heals wounds, certainly raw honey (unheated & unfiltered) which contains Lactic Acid Bacteria. Many studies have demonstrated its healing capacity with regards to wounds but the researchers chose hard-to-heal wounds that took more than a year to heal! The results are very impressive, as antibiotic-resistant strains are becoming more commonplace in medical establishments.

Fighting Off Wound Pathogens in Horses with Honeybee Lactic Acid Bacteria
Current Microbiology October 2016

In the global perspective of antibiotic resistance, it is urgent to find potent topical antibiotics for the use in human and animal infection. Healing of equine wounds, particularly in the limbs, is difficult due to hydrostatic factors and exposure to environmental contaminants, which can lead to heavy bio-burden/biofilm formation and sometimes to infection. Therefore, antibiotics are often prescribed. Recent studies have shown that honeybee-specific lactic acid bacteria (LAB), involved in honey production, and inhibit human wound pathogens.

The aim of this pilot study was to investigate the effects on the healing of hard-to-heal equine wounds after treatment with these LAB symbionts viable in a heather honey formulation. For this, we included ten horses with wound duration of >1 year, investigated the wound microbiota, and treated wounds with the novel honeybee LAB formulation. We identified the microbiota using MALDI-TOF mass spectrometry and DNA sequencing. In addition, the antimicrobial properties of the honeybee LAB formulation were tested against all wound isolates in vitro.


Our results indicate a diverse wound microbiota including fifty-three bacterial species that showed 90 % colonization by at least one species of Staphylococcus. Treatment with the formulation promoted wound healing in all cases already after the first application and the wounds were either completely healed (n = 3) in less than 20 days or healing was in progress. Furthermore, the honeybee LAB formulation inhibited all pathogens when tested in vitro.


Consequently, this new treatment option presents as a powerful candidate for the topical treatment of hard-to-heal wounds in horses. 

The rapid, painless healing of hard-to-heal equine wounds gives us reason to believe that the honeybee LAB formulation presents a new topical option in future wound healing. This new treatment may be a stepping-stone toward an alternative solution for treating other infected wounds in animals and humans and warrants further investigation.


Friday, August 12, 2016

Chrysin Flavonoid in Honey, Propolis Prevents Cell Damage

Just one of numerous flavonoids found in propolis and in smaller doses in honey, Chrysin has been found to protect cells from lab-induced ammonia intoxication. The protective effects of this flavonoid is a common trait with propolis, where we find it always protecting and healing various conditions as well as preventing damaging effects from chemicals, drugs, pesticides, etc.

Biomed Pharmacother. 2016 May 24;82:345-354

Chrysin (5,7-dihydroxyflavone) is a major component of some traditional medicinal herbs present in honey, propolis and many plant extracts.

The study was aimed to illuminate the effect of chrysin in the pathogenesis of ammonium chloride (NH4Cl) induced hyperammonemic rat model in a dose dependent manner. Rats were injected with NH4Cl (100mg/kg b.w.) by intraperitonially (i.p) thrice a week for 8 consecutive weeks for the induction of experimental hyperammonemia. Hyperammonemic rats were treated with chrysin by orally at a dose of 25, 50 & 100mg/kg b.w. respectively. 

Protective effect of chrysin against hyperammonemia was evaluated by performing biochemical estimations and morphopathological investigations of hematoxylin and eosin stained sections of liver, brain and kidney tissues. Supplementation of chrysin reinstated the levels of blood ammonia, plasma urea, uric acid, total bilirubin, creatinine, brain glutamate, glutamine, nitric oxide (NO) and the activities of Na+/K+-ATPase, and liver marker enzymes.
On the other hand increased level of plasma urea was observed in chrysin treated rats as compared with hyperammonemic rats. Chrysin administration caused distortion of hepatic, brain and kidney architecture as shown by histological examination.

Chrysin at a dose (100mg/kg b.w.) showed an utmost decline in the level of all biochemical estimations. Both biochemical and morphological studies clearly revealed that chrysin protects against cell injury induced by ammonia intoxication in a dose-response manner with respect to endogenous antioxidants and hypoammonemic effects.


Wednesday, July 20, 2016

Chronic Rhinosinusitis Healed with Propolis

A really good study confirming the beneficial effects of propolis for chronic sinus ailments such as rhinosinusitis. Another interesting point is the consistent effect of its healing, protective properties on injuries, especially in mucous linings of the body.

Chronic Rhinosinusitis Healed with Propolis


INTRODUCTION:
Mechanical trauma to the nasal mucosa increases the risk of synechia formation, especially after chronic rhinosinusitis and nasal surgeries.

OBJECTIVE:
This study was carried to assess the effect of propolis administration in healing injured nasal mucosa in rats.

METHODS:
We randomly divided eighteen rats into three equal experimental groups: (1) non-treated group; (2) gum tragacanth (suspending agent for propolis) treated group; and (3) propolis treated group. The non-treated group received no treatment for 15 days. The second group received gum tragacanth administration (5 ml/kg, orally) once daily for 15 days. The third group received propolis suspension orally at a dose of 100 mg/kg once daily for 15 days. At the beginning of this study, we induced unilateral mechanical nasal trauma on the right nasal mucosa of all rats in the three groups using a brushing technique. A pathologist stained tissue samples using hematoxylin and examined eosin by using a light microscope.
Photomicrographs of (1) non-treated, (2) gum (3) propolis

RESULTS:
The severity of inflammation was milder with the absence of ulcerations in the propolis treated group compared with the non-treated and gum tragacanth groups. Goblet cell and ciliated cell loss was substantially lower in patients treated with propolis compared with groups without treatment and those treated with gum tragacanth.

CONCLUSION:
Propolis decreased inflammation and enhanced healing of wounds of the nasal mucosa in rats.

   

Monday, June 13, 2016

Propolis Reduces Risk of Cataracts

This eye-opening study provides evidence of another benefit from the super antioxidant power of propolis. This is important news for those suffering from diabetes, as sugar cataracts are common. Previous studies have also determined propolis to be a safe antidiabetic alternative for diabetics to consume propolis even at 50-200mg/kg/BW. 

Propolis, a Constituent of Honey, Inhibits the Development of Sugar Cataracts and High-Glucose-Induced Reactive Oxygen Species in Rat Lenses
J Ophthalmol. 2016, April 19;2016:1917093


Purpose 
This study investigated the effects of oral propolis on the progression of galactose-induced sugar cataracts in rats and the in vitro effects of propolis on high-glucose-induced reactive oxygen species (ROS) and cell death in cultured rat lens cells (RLECs).

Methods 
Galactose-fed rats and RLECs cultured in high glucose (55 mM) medium were treated with propolis or vehicle control. Relative lens opacity was assessed by densitometry and changes in lens morphology by histochemical analysis. Intracellular ROS levels and cell viability were measured.

Results 
Oral administration of propolis significantly inhibited the onset and progression of cataract in 15% and 25% of galactose-fed rats, respectively. RLECs cultured with high glucose showed a significant increase in ROS expression with reduced cell viability. Treatment of these RLECs with 5 and 50 μg/mL propolis cultured significantly reduced ROS levels and increased cell viability, indicating that the antioxidant activity of propolis protected cells against ROS-induced damage.
figure 2


Conclusion 
Propolis significantly inhibited the onset and progression of sugar cataract in rats and mitigated high-glucose-induced ROS production and cell death. These effects may be associated with the ability of propolis to inhibit hyperglycemia-evoked oxidative or osmotic stress-induced cellular insults.



Sunday, May 22, 2016

Allergy Relief Effective with Propolis

This study reaffirms once again, the holistic effects of propolis on mucous linings of the body, in particular the nose. Its anti-inflammatory properties also provide anti-allergic effects, ideal for those who suffer from respiratory ailments. Just another amazing product made by honeybees.

Effects of propolis in an experimental rat model of allergic rhinitis








Purpose
The aim of this study was to determine the anti-allergic activity of propolis in an ovalbumin-induced rat model of allergic rhinitis.

Materials and methods
This prospective experimental study was conducted at Hakan Çetinsaya Clinical and Experimental Animal Research Center with 30 rats. After sensitization of all rats with 0.3 mg intraperitoneal ovalbumin plus 30 mg aluminum hydroxide for 14 days (first phase), rats were divided to five groups. In the second phase of the study 10 μL of ovalbumin was applied to each nostril for 21 days. Together with second phase, ketotifen (n:6), oral propolis (n:6), intranasal propolis (n:6) and intranasal mometasone furoate (n:6) were given to rats. A control group (n:4)(salin) and sham group (n:2) were planned. Symptoms were assessed on days 19, 22, 25, 30 and 35, resulting in 5 symptom scores: symptom scores 1–5. On day 35, nasal tissue was removed and histological examination was performed.

Results
When rats that received systemic and intranasal propolis were compared to controls, ciliary loss, inflammation, increase in goblet cells, vascular proliferation, eosinophil count, chondrocytes and allergic rhinitis symptom score were found to be decreased (p < 0.05).

Conclusions

It was found that propolis had anti-allergic effects on allergic symptom scores and nasal histology.