Tuesday, October 14, 2014

Propolis Detoxifies Cancer Drug Side Effects

A consistent thread in all studies involving bee propolis is its complementary effects, whether it enhances the drug or protects the body from negative side effects. This study determined that propolis detoxified the liver and kidney from the toxic effects of this cancer drug. Ironically, propolis is also proven to enhance the tumor destructive power of breast cancer medication. It would be well-recommended for anyone preparing to undergo chemotherapy, to take propolis daily before, during and after treatment...

Ameliorative effect of propolis against cyclophosphamide-induced toxicity in mice
Pharm Biol, 2014 Oct 7:1-7. [Epub ahead of print]

Context
Cyclophosphamide (CTX) is a common anticancer agent used for the treatment of several malignancies. However, upon treatment, it induces severe toxicity due to its oxidative stress capability. Propolis, a natural product collected by honey bees, has shown several biological activities, such as free radical scavenging and antioxidant agent.

Objective
This study elucidates the protective effects of propolis against CTX-induced changes in mice. Materials and methods: Forty-eight male Swiss albino mice were divided into four groups; group 1 was intraperitoneally (i.p.) injected with 200 µL of phosphate buffer saline (PBS), group 2 was injected with 100 mg/kg/d propolis, group 3 was injected with a single dose of CTX (200 mg/kg), and group 4 was injected with a single dose of CTX (200 mg/kg) followed by propolis (100 mg/kg) for 7 consecutive days. After 12 d, mice were bled and then sacrificed to analyze the hematological, biochemical, and histological parameters.

Results
The results indicated that CTX-injected mice showed an increase in the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), urea, and creatinine and a decrease in the total number of white blood cells (WBCs) and platelets. Moreover, dramatical changes in the histological architectures of the liver and kidney were observed. The mice that were injected with CTX/propolis showed an improvement in the levels of ALT, AST, urea, creatinine, WBCs, and platelets. Moreover, the histological picture of the liver and kidney was significantly improved.


Conclusions
In conclusion, propolis might be considered an effective agent in ameliorating the toxicity resulted from CTX treatment.


Monday, September 29, 2014

Honey Outperforms Sugars As Anti-Cancer Agent

This study confirms that the holistic capacity of honey, which is rich in sugars, cannot be duplicated to possess the same anticancer effects as lab-created honey. Honey has unique origins from the nectar sources to the transitional processes that honey bees use to make it medicinal and nutritional. Honey is good medicine.

EFFECT OF SUGAR CONCENTRATION MIMICKING COMPOSITION OF SUGARS IN HONEY ON N-METHYL-N-NITROSOUREA (MNU)-INDUCED BREAST CARCINOMA IN RATS
Pathology: October 2014 - Volume 46

Aim
High concentration of sugar is carcinogenic. Honey which is rich in sugars has been shown to have anti-cancer effect. There is no study reported on the effect of sugars mimicking the concentration of sugars of honey (honey-mimic) in inhibiting breast carcinoma in rats. The aim of the study is to investigate if honey-mimic has similar effect as natural honey on experimental breasts tumor induced by N-methyl-N-nitrosourea (MNU) in rats.

Methods
Honey-mimic was made by mixing proportions of fructose, glucose, sucrose and maltose mimicking the sugar composition of honey based on published method. Forty Sprague-Dawley rats were randomly divided into 4 groups with 10 animals per each group. Group 1 rats did not receive MNU injection (negative control). Group 2, 3, 4 rats received a single intraperitoneal dose (80 mg/kg body weight) of MNU injection. The size and development of the tumors were monitored. When the tumor size reached 10-12 mm2 in diameter, rats of Group 3 were given oral honey-sugar mimic 1.0 g/kg while Group 4 was given honey 1.0 g/kg body weight daily. Group 2 was not given honey-sugar mimic nor natural honey (positive control). All rats were allowed to feed on rat chowder ad lib. After 120 days, all rats were sacrificed and tumors were harvested for gross and histopathological examinations
.
Results
The mean number of tumors developed per rat in groups 2, 3, 4 was 4.9 +/- 0.60, 3.6 +/- 0.40 and 3.4 +/- 0.30, respectively. The mean tumor weight and volume in the negative and positive control group were significantly larger at 11.85 +/- 1.01 g and 8.50 +/- 0.40 cm3, respectively; while in the test groups (Groups 3 and 4) were 6.45 +/- 0.60 g and 4.50 +/- 0.20 cm3; 4.34 +/- 0.35 g and 2.50 +/- 0.20 cm3 (p < 0.05), respectively. Histopathological grading revealed that the majority of rats which received honey-sugar mimic and honey were of grade 1 and 2 compared to control, which were of grade 3.

Conclusion
Sugar concentration mimicking composition of sugars in natural honey has some anti-carcinogenesis modulation properties but not as effective as honey.


Monday, September 22, 2014

Study Confirms Propolis is a Safe, Antidiabetic Agent

This is an amazing discovery for diabetics, as the use of propolis aids the body by regulating the immune system to protect it. As it's been described, honey bees make propolis from a naturopathic formula, which protects the liver, pancreas and kidneys. 

Significance of propolis administration for homeostasis of CD4+CD25+ immunoregulatory T cells controlling hyperglycemia
Springer Plus, 2014 Sept 15; 3:526  

In the present study, we examined the effect of ethanolic soluble derivative of propolis (EEP) extract on immunological function in diabetic mouse models with the aim of highlighting the role of regulatory T cell, the change of cell surface molecule, and in vivo productions of IFN-gamma. Murine models of diabetes mellitus (DM) were created by injecting normal mice with S961 peptide. 

Normal BALB/c mice were injected intraperitoneally with peptide S961 300 mg/kg body weight (BW) twice a day for eight days. On day 15, the spleen was isolated; then, cell surface molecules and regulatory T cells were analyzed using flow cytometry. 

The histopathological changes were monitored in the liver of treated and control mice. Afterward, we tested the ability of propolis as an immunomodulator that initiate normality metabolism and homeostasis. 

The propolis decreased blood sugar levels and increased the number of naive T cells expressing CD62L molecule by suppressing the development of effector cells in diabetic mice. However, the propolis stimulated development of effector cells, which was indicated by increasing the number of CD4+CD25+ T cells in normal mice. 

Moreover, the propolis increased the production of IFN-gamma in normal mice, whereas in mouse models of diabetes mellitus propolis tends to suppress the production of IFN-gamma. The histological analysis of the liver shows that at a dose of 50-200 mg/kg BW propolis does not show a toxic effect so that the dose is categorized safe.

Therefore, the ethanolic soluble derivative of propolis (EEP) extract warrant for further exploited as an antidiabetic agent that safe for human.


Click here to download complete study.



Monday, September 15, 2014

PMS Reduced by Consuming Royal Jelly

Royal jelly is a hormone-rich substance that is encouraged for women, whether during pregnancy or in menopause. This triple-blind study reinforces the benefits women gain when consuming royal jelly to diminish PMS. Studies dating back to the 70's, identified that a blend of bee pollen and royal jelly reduced symptoms of PMS and menopause. In fact, royal jelly plays an important role in regulating hormones, not only for women, but for men as well. 

Effect of Royal Jelly on premenstrual syndrome among Iranian medical sciences students: A randomized, triple-blind, placebo-controlled study
Complement Ther Med. 2014 Aug;22(4):601-6

BACKGROUND:
Premenstrual syndrome (PMS) may have negative effects on women's health and sometimes need therapeutic non-pharmacological management.

OBJECTIVES:
To determine the effect of oral consumption of 1000mg Royal Jelly capsule on premenstrual syndrome.

METHOD:
This is a randomized, triple-blind, placebo-controlled clinical trial, which was conducted in Tehran University of Medical Sciences female dormitories between December 2011 and October 2012. The study population comprised 110 medical sciences student with PMS, whom were randomly assigned to an intervention or a control group. Each participant in the intervention group took one Royal jelly capsule orally per day, starting on the first day of menstruation and continued the same treatment daily throughout two consecutive menstrual cycles, while participants in the intervention group took placebo capsules as same method. The outcome measure in this study was PMS score as obtained through the Premenstrual Profile 2005.

RESULTS:
The mean of personal characteristics and baseline level of the premenstrual score before intervention did not differ between groups. After two consecutive months consumption of Royal Jelly, PMS score had decreased from 23.17±17.43 to 11.42±14.58 (mean change: 11.75; 95% confidence interval [CI]: 8.31-15.19) and in Placebo, PMS score changed from 21.48±16.39 to 20.27±15.76 (mean change: 1.20; 95% CI: -1.69 to 4.10). Also difference between mean changes was significant (mean difference: 10.54; 95% CI: 6.10-14.98).

CONCLUSIONS:
The results of the study have demonstrated that 2 months consumption of Royal Jelly was effective in reducing PMS

Monday, September 8, 2014

Reduced Toxicity Achieved in Liver, Spleen & Pancreas with Apitherapy

A fantastic study revealing the "synergistic effects" of an Apitherapy diet, combining multiple bee products enhances the effects of each individual product thereby boosting the overall results. This study reaffirms the previous results which identified the detoxing effects of honey and propolis on liver and kidneys.

Effect of Apitherapy Formulations against Carbon Tetrachloride-Induced Toxicity in Wistar Rats after Three Weeks of Treatment
Molecules 2014, 19(9), 13374-13


The human body is exposed nowadays to increasing attacks by toxic compounds in polluted air, industrially processed foods, alcohol and drug consumption that increase liver toxicity, leading to more and more severe cases of hepatic disorders. The present paper aims to evaluate the influence of the apitherapy diet in Wistar rats with carbon tetrachloride-induced hepatotoxicity, by analyzing the biochemical determinations (enzymatic, lipid and protein profiles, coagulation parameters, minerals, blood count parameters, bilirubin levels) and histopathological changes at the level of liver, spleen and pancreas.
Liver samples

The experiment was carried out on six groups of male Wistar rats. Hepatic lesions were induced by intraperitoneal injection of carbon tetrachloride (dissolved in paraffin oil, 10% solution). Two mL per 100 g were administered, every 2 days, for 2 weeks. Hepatoprotection was achieved with two apitherapy diet formulations containing honey, pollen, propolis, Apilarnil, with/without royal jelly.
Spleen samples

Biochemical results reveal that the two apitherapy diet formulations have a positive effect on improving the enzymatic, lipid, and protein profiles, coagulation, mineral and blood count parameters and bilirubin levels. The histopathological results demonstrate the benefits of the two apitherapy diet formulations on reducing toxicity at the level of liver, spleen and pancreas in laboratory animals.
Pancreas samples

To download the full whitepaper, click here.


Sunday, August 31, 2014

Propolis Detoxifies Kidney of Aluminum Chloride

Yet another study confirming the protective effects of propolis, detoxifying kidneys from aluminum chloride. Other studies have shown its ability to detoxify the liver. This characteristic is also seen with consuming honey which aids in detoxifying the liver. Bee products are protective with no negative side effects and propolis is one of the most important products from honeybees.

Role of propolis (bee glue) in improving histopathological changes of the kidney of rat treated with aluminum chloride
Environ Toxicol. 2014 Sep;29(9):1000-10

Humans are frequently exposed to aluminum from various food additives, therapeutic treatments and the environment, and it can be potentially toxic. This study is aimed to elucidate the protective effects of propolis against aluminum chloride (AlCl3 )-induced histopathological and immunohistochemical changes in kidney tissues of rats. 

Sixty Wistar Albino male rats (average weight 250-300 g) were divided into three equal groups. The first served as a negative control. The second received AlCl3 (34 mg/kg bw, 1/ 25 LD 50). The third were administered AlCl3 (34 mg/kg bw, 1/ 25 LD 50) plus propolis (50 mg/kg bw). Doses were given once daily via a gavage for 8 weeks every day. The results showed that shrunken glomeruli, intraglomerular congestion, loss of apical microvilli, degeneration of mitochondria and widened rough endoplasmic reticulum were also observed in the Proximal Convoluted Tubules of these animals.

Treatment with propolis ameliorated the harmful effects of AlCl3 ; this was also proved histopathologically by the noticeable improvement in the renal tissues. There were also significant variations in the expressed of ki-67 and p53 proteins. It can be concluded that propolis may be promising as a natural therapeutic agent in AlCl3 -induced renal toxicity and oxidative stress in rat kidneys.

Monday, August 25, 2014

Neurodegenerative Diseases Prevented by Bee Venom

Bee venom and its numerous components are revealing many uses in treating health conditions. Incorporating other bee products during such a treatment would generate additional benefits, namely "synergistic effects", whereby each product enhances the properties of the others, such as royal jelly & propolis.

Neuroprotective effects of melittin on hydrogen peroxide-induced apoptotic cell death in neuroblastoma SH-SY5Y cells
BMC Complementary and Alternative Medicine 2014, 14:286, Published: 5 August 2014
Background
Free radicals are involved in neuronal cell death in human neurodegenerative diseases. Since ancient times, honeybee venom has been used in a complementary medicine to treat various diseases and neurologic disorders. Melittin, the main component of honeybee venom, has various biologic effects, including anti-bacterial, anti-viral, and anti-inflammatory activities.

Methods
We investigated the neuroprotective effects of melittin against H2O2-induced apoptosis in the human neuroblastoma cell line SH-SY5Y. The neuroprotective effects of melittin on H2O2-induced apoptosis were investigated using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenylterazolium bromide assay, caspase 3 activity, 4,6-diamidino-2-phenylindole staining, a lactate dehydrogenase release assay, Western blots, and reverse transcription-polymerase chain reaction.

Results
The H2O2-treated cells had decreased cell viability with apoptotic features and increased production of caspase-3. On the other hand, melittin treatment increased cell viability and decreased apoptotic DNA fragmentation. Melittin attenuated the H2O2-induced decrease in mRNA and protein production of the anti-apoptotic factor Bcl-2. In addition, melittin inhibited both the H2O2-induced mRNA and protein expression of Bax-associated pro-apoptotic factor and caspase-3.

Conclusions

These findings suggest that melittin has potential therapeutic effects as an agent for the prevention of neurodegenerative diseases.