Studies have already proven honey to be an effective medium for human tissue but this study confirms that it not only improves human sperm performance of infertile patients but also protects them after a deep freeze of 6 months!
Honey Supplementation to Semen-Freezing Medium Improves Human Sperm Parameters Post-Thawing
J Family Reprod Health, 2014 Mar
To evaluate the effect of honey supplemented to cryoprotectant medium on post-thaw sperm motility, concentration, morphology and agglutination.
MATERIALS AND METHODS:
Thirty semen samples were collected from 30 infertile patients. After assessment of semen analysis, semen samples were divided into 3 aliquots (0.7ml for each) and mixed with 1 ml of cryopreservation solution (G1, control) alone, or enriched with 5% honey (G2) or with 10% honey (G3) for cryopreservation. Cryopreservation was done at -196°C in liquid nitrogen and thawing was performed after six months. Direct swim up technique was used for in vitro sperm preparation post-thawing. Sperm parameters were assessed and data were statistically analyzed pre- and post-thawing.
Results appeared that the percentage of sperm motility for G1 and G2 groups were significantly reduced (P < 0.05) post-thawing when compared to pre-cryopreservation. However, there was no significant difference in the total motility (%) of the post-thaw sperm between the G1 and G2 groups. While there was significant increased (P < 0.05) in the percentage of normal sperm morphology for G1 and G3 groups post-thawing. Post-thawing normal sperm morphology (%) for G3 group was significantly increased (P < 0.05) as compared to G1 and G2 groups. In contrast non significant differences (P > 0.05) were observed between G1 and G2 groups. Significant reduction (P < 0.05) was seen in the sperm concentration for all groups post-thawing as compared to pre-cryopreservation groups. After thawing the results reveal significant reduction (P < 0.05) in the sperm agglutination (%) for G3 group as compared to G1 and G2 groups.
CONCLUSION:The results of this study indicated that the supplementation of honey (10%) to cryoprotectant solution results in enhancement of sperm quality post-thawing.