It's well-known that the synergistic properties of bee products are amplified when taken together. In this case, they pulled a phenolic component of honey, caffeic acid, which is also a primary component in propolis, to suppress cancer cell growth. This study reinforces the prophylactic effect of taking bee products daily...
Growth
Inhibition by Caffeic Acid, One of the Phenolic Constituents of Honey, in HCT
15 Colon Cancer Cells
Scientificité Journal, 2012 Apr 29
Previous
work from our laboratory showed that the mechanism of crude-honey induced
apoptosis in colon cancer cells. Since phenolic constituents of honey were
attributed to its apoptosis-inducing ability, we studied caffeic acid, one of the
phenolic constituents of honey, induced effect on colon cancer cells.
Antiproliferative
effect of caffeic acid was estimated using
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. MTT
assay signified the antiproliferative nature of caffeic acid against the HCT 15
colon cancer cells.
A
time-dependent inhibition of colony formation was evident with caffeic acid
treatment. Cell-cycle analysis of caffeic acid- (CA-) treated cells indicated increasing
accumulation of cells at sub-G(1) phase. Photomicrograph images of treated
cells showed membrane blebbing and cell shrinkage. Yo-pro-1 staining of
caffeic-acid-treated cells confirmed apoptosis in dose- and time-dependent
manner. Increasing ROS generation and reduction in the mitochondrial membrane
potential were also accompanied in the caffeic acid-induced apoptosis.
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