Propolis is usually identified as having strong anti-fungal properties. However, this study finds a positive coorrelation in using the synergistic antifungal properties found in honey and curcuma, to create a cost-effective, Api-Phytotherapeutic treatment to combat antifungal resistance...
In vitro activity of natural honey alone and in combination with curcuma starch against Rhodotorula mucilaginosa in correlation with bioactive compounds and diastase activity
Asian Pac JTrop Biomed, 2013 Oct
To evaluate the in vitro activity and synergism of the combinations of natural honey and curcuma starch against Rhodotorula mucilaginosa in correlation with total phenolic, flavonoid contents, and diastase activity.
The Folin-Ciocalteu test was used to determine the total polyphenols content and the flavonoid content was analyzed using by the aluminum chloride method. The antifungal activity of the natural honey, determined by an agar well diffusion assay and agar incorporation method.
Total phenolic content varied from (63.930.11) to (95.366.08) mg GAE/100 ghoney as gallic acid equivalent. Total flavonoids content varied from (5.41±0.04) to (9.94±0.54) mg CE/100 g. Diastase activity values were between (7.3±2.8) and (26±2.8). The zone inhibition diameter for the six honey samples without starch ranged between 6 and 20 mm. When starch was mixed with honey and then added to well, a zone inhibition increase diameter 7 and 21 mm. The percentage increase was noticed with each variety and it ranged between 5% and 62.5%. The minimal inhibitory concentrations for the
CONCLUSIONS:The mixture of curcuma starch and honey could lead to the development of new combination antibiotics against Rhodotorula infections.