Important news from a study which finds the anti-cancer capacity of bee venom is effectively enhanced when mixed at a lower dosage in a culture of white blood cells. Interestingly enough, this anti-apoptopic effect (cell death) is also found true in propolis. Bee products are definitely pro-life...
Co-culture
with NK-92MI cells enhanced the anti-cancer effect of bee venom on NSCLC cells
by inactivation of NF-Κb
Arch Pharm
Res, 2014 Jan 1
In the
present study we experimented on a multimodal therapeutic approach, such as
combining chemotherapy agent (Bee venom) with cellular (NK-92MI) immunotherapy.
Previously bee venom has been found to show anti-cancer effect in various
cancer cell lines. In lung cancer cells bee venom showed an IC50 value of 3 μg/ml in both cell lines. The
co-culture of NK-92MI cell lines with lung cancer cells also show a decrease in
viability up to 50 % at 48 h time point. Hence we used bee venom treated NK-92MI
cells to co-culture with NSCLC cells and found that there is a further decrease
in cell viability up to 70 and 75 % in A549 and NCI-H460 cell lines
respectively.
We further
investigated the expression of various apoptotic and anti-apoptotic proteins
and found that Bax, cleaved caspase-3 and -8 were increasing where as Bcl-2 and
cIAP-2 was decreasing. The expression of various death receptor proteins like
DR3, DR6 and Fas was also increasing. Concomitantly the expression of various
death receptor ligands (TNFalpha, Apo3L and FasL) was also increasing of
NK-92MI cells after co-culture. Further the DNA binding activity and luciferase
activity of NF-κB was
also inhibited after co-culture with bee venom treated NK-92MI cell lines. The
knock down of death receptors with si-RNA has reversed the decrease in cell
viability and NF-κB
activity after co-culture with bee venom treated NK-92MI cells.
Thus this
new approach can enhance the anti-cancer effect of bee venom at a much lower
concentration.
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