This study finds Bee Venom has the potential to advance wound healing practices and we find yet another medicinal use for honey bee products. From these results, it appears one could combine bee venom, propolis and honey to create a super wound healing agent...
Effects of honeybee (Apis mellifera) venom on keratinocyte migration in vitro
Pharmacogn Mag, 2013, July 9
Since the ancient times the skin aging application of honeybee venom (BV) is practiced and persisted until nowadays. The present study evaluated the effect of the honeybee venom (BV) on keratinocyte migration in wound healing model in vitro.
To access BV further as a cosmetic ingredient and a potential external application for topical uses, we performed studies to investigate the biologic effect of BV treatment on keratinocyte proliferation and migration in vitro.
MATERIAL AND METHODS:
BV cytotoxicity was assessed by using a 3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl tetrazolium bromide (MTT) assay over 24 h. To assess BV genotoxicity, damage to human epidermal keratinocyte (HEK) was evaluated using the Comet assay. HEK migration was evaluated using a commercial wound healing kit. The skin pro-inflammatory cytokines interleukin (IL)-8 and tumor necrosis factor (TNF)-α were examined to evaluate the pro-inflammatory response to BV.
|BV effect on keratinocyte migration|
It was found that BV [< 100 ug/ml] was not cytotoxic and stimulated more HEK proliferation and migration compared to negative control, and did not induce DNA damage. There were also decreases in IL-8 and TNF-α expression levels in HEK at all time points.
These findings highlight the potential of topical application of BV for promoting cell regeneration and wound treatment.