Propolis Found to Inhibit Candida Albicans

The antifungal protection of Propolis is due to its rich, diverse flavonoid content and it's proven control of Candida Albicans has been validated in studies worldwide...

Structural and Genetic Alterations of Fungal Cells Caused by Mexican Propolis

Science against Microbial Pathogens: Communicating Current Research and Technological Advances, 2011, VOL. 2

In order to study the antifungal activity of propolis ethanol extracts (PEE) from Apis mellifera bees from Mexico we used optical microscopy and we were able to see that PEE induced inhibition of germ tube formation of C. albicans.


Ultrastructural findings were seen using transmission electron micrographs of ultrathin sections of Candida albicans revealed that these cells suffered vacuolization, increased formation of storage granules as well as alteration and disruption of the outer structures of yeast, with release of intracellular material. In order to describe the molecular mechanism of action, we evaluate gene expression...

We were able to identify some over- and under-expressed genes. ADH1 y PIK1 genes were under-expressed in a manner dependent on concentration and exposure time. On the other hand, our team has determined the chemical profile of several Mexican propolis, with interesting results as the diversity of content and different levels of antifungal and antibacterial activities…

Although many studies have focused on demonstrating the antifungal activity of propolis and different extracts, few have shown their effects on the morphology and structure of fungi. We have found important cell damage caused by contact with extracts using bright field optical microscopy studies as well as the fluorescent dye White Calcofluor and transmission electron microscopy...

Based on the above results, it can be stated that propolis from the States of Guanajuato and Queretaro showed a higher number of compounds with longer retention time (higher polarity), which was corroborated by the MS study, in which it was found that these compounds were of the flavonoid-type, mainly pinocembrin, naringenin and chrysin, compounds characteristic of plant specimens visited by bees in that geographic locality...

In summary, we succeeded in establishing the chemical content of propolis, with a significant presence of flavonoid type compounds, a prerequisite for such high activity. Similarly, we must remember that the characteristic flora of each region determines the type of propolis bees collect in that locality, and this accounted for the differences in the antimicrobial activity which we found in this study.

Honey Protects Against Sexual Dysfunction Induced by Cigarettes

Yet another beneficial advantage to eating honey, this study shows the merits of eating honey for prophylactic protection, even if you aren't a smoker...

Protective Effect of Honey Against Cigarette Smoke Induced-Impaired Sexual Behavior and Fertility of Male Rats

Toxicol Ind Health, 2012 Jan 24

Cigarette smoking is associated with sexual dysfunction and impaired fertility in males. The aim of this study was to determine the potential protective effect of honey against the toxic effect of cigarette smoke (CS) on sexual behavior and fertility of male rats.

Thirty-two adult Sprague-Dawley rats were randomly divided into four groups (8 rats/group) as control, honey (H), CS and H plus CS (H + CS) groups. Rats in control and CS groups received oral administration of distilled water daily while rats in H and H + CS groups received honey (1.2 g/kg body weight/day) by oral gavage. Rats in CS and H + CS groups were also exposed to CS for 8 min 3 times/day. From 10 to 13 weeks of treatment, each male rat was cohabited with 3 untreated female rats for sexual behavioral and reproductive performance studies.

Honey significantly increased the percentages of rats achieving intromission and ejaculation as well as increased mating and fertility indexes of male rats exposed to CS. Thus, honey has a protective effect against CS-induced impaired sexual behavior and fertility in male rats.

Diabetic Foot Ulcers Healed With Royal Jelly

With over 185 organic compounds identified in Royal Jelly, is it any wonder that it possesses the same wound healing capacity as honey...

The Efficacy of Topical Royal Jelly on Diabetic Foot Ulcers Healing: A Case Series

J Res Medical Sciences, 2011 July

BACKGROUND:

Foot ulcers and infections are the major sources of morbidity in individuals with diabetes mellitus. This study aimed to evaluate the efficacy of topical Royal Jelly (a worker honey bee product) on healing diabetic foot ulcers.

METHODS:

Diabetic patients with foot ulcers that were referred to our clinic at Khorshid Hospital, Isfahan, Iran; were evaluated three times a week and treated according to standard treatments consisted of offloading, infection control, vascular improvement and debridement if required. In addition, all ulcers were measured and then topical sterile 5% Royal Jelly was applied on the total surface area of the wounds. Eventually, they were covered with sterile dressings. Each patient was followed for a period of three months or until the complete healing.

RESULTS:

A total of eight patients were enrolled in this study. Of these, two had two ulcers and, therefore, ten ulcers were evaluated. Two ulcers were excluded. Seven of the remained eight ulcers healed. Mean duration of complete healing was 41 days. One ulcer did not completely heal but improved to 40% smaller in length, 32% in width and 28% in depth. The mean length, width and depth reduction rates were 0.35 mm/day, 0.28 mm/day and 0.11 mm/day, respectively.

CONCLUSIONS:

Royal Jelly dressing may be an effective method for treating diabetic foot ulcers besides standard treatments.

Propolis, Modulator for Immune & Inflammatory Responses

Propolis is truly a remarkable therapeutic agent, proven here to be an effective cellular regulator, independently and with its complementary co-phyto ingredients...

The Effects of Propolis and Its Isolated Compounds on Cytokine Production by Murine Macrophages

PhytotherapyResearch, 25 January 2012

Since propolis and phenolic compounds, such as cinnamic and coumaric acids, have several biological properties, their immunomodulatory effect on cytokine production (IL-1β, IL-6 and IL-10) was investigated.

Peritoneal macrophages from BALB/c mice were incubated with propolis, coumaric and cinnamic acids in different concentrations and the concentrations that inhibited cytokine production were tested before or after macrophage challenge with LPS, to evaluate a possible immunomodulatory action. Propolis and the acids stimulated IL-1β production, while IL-6 production was significantly inhibited after incubation with propolis (5, 50 and 100 µg/well), coumaric and cinnamic acids (50 and 100 µg/well).

In LPS-challenge protocols, inhibitory concentrations of cinnamic and coumaric acids after LPS incubation prevented efficiently its effects on IL-6 production, whereas propolis inhibited LPS effects both before and after its addition. Propolis, coumaric and cinnamic acids (50 and 100 µg/well) inhibited IL-10 production as well. Both acids showed a similar inhibitory activity on IL-10 production when added after LPS challenge, while propolis counteracted LPS action when added before and after LPS incubation.

Propolis modulated the immune/inflammatory response, depending on the concentration. Its efficiency may occur due to the synergistic effect of its compounds, and cinnamic and coumaric acids may be involved in the action of propolis on cytokine production.

Researchers Identify 185 Organic Compounds in Royal Jelly

The super-tonic effects of royal jelly are identified chromatically, along with their physiological benefits as a bactericide, a source of free amino acids and storage requirements for fresh royal jelly...

Gas Chromatographic-Mass Spectrometric Investigation of Volatile and Extractable Compounds of Crude Royal Jelly

J ChromatogrB Analyt Technol Biomed Life Sci, 2011 Dec 30

Using headspace solid-phase microextraction (HS-SPME) followed by diethyl ether and methanol extraction, it was possible to isolate as many as 185 organic compounds out of 17 samples of crude royal jelly (RJ). Of the above compound number, 169 compounds were positively identified by means of gas chromatography-mass spectrometry.

The volatile fraction of RJ consists of 25 different compounds where approximately 47% of the total ion current (TIC) of volatile compound chromatograms were composed of substances characterized by bactericidal (phenols) and repelling (octanoic acid and 2-heptanone) activities. Preliminary investigations have shown that RJ stored for 10 months at -18°C and 4°C keeps its composition of volatile compounds unchanged, however, at the same time at room temperature RJ phenol contents is decreased twice, whereas the fraction of aliphatic acids is increased 2.8 times due to the presence of both acetic and butyric acids.

The chromatogram of RJ ether extracts showed 85 different compounds, however about 88% of TIC consisted exclusively of 8 compounds, i.e. 10-hydroxy-2-decenoic, 10-hydroxydecanoic, 3,10-dihydroxydecanoic, 8-hydroxyoctanoic, 2-decene-1,10-dioc and (Z)-9-hydroxy-2-decenoic acids. Nine aliphatic acids, which were detected for the first time, are the homologues of hydroxy- and oxo-acids identified earlier in RJ. In the RJ methanol extracts 82 compounds were identified, mainly carbohydrates and their derivatives.

Approximately 87% of TIC consisted of fructose, glucose and sucrose. Special attention was paid to discrepancies between obtained and literature data concerning the presence of free amino acids in RJ. It was suggested that these inconsistencies can be explained by the differences in the methods of RJ collection and/or sample preparation.

Conclusion:

► Volatile compounds of fresh royal jelly. 

► Bactericidal and repelling activities. 

► Markers of inappropriate storage. 

► Extractable compounds of royal jelly. 

► Possible source of free amino acids.

Polyphenols Needed for Honey Antibacterial Effect

Why does honey heal wounds? Its antibacterial activity is due to its ability to produce Hydrogen Peroxide and now this Canadian study identifies the cause. Thanks to honeybees collecting nectar from flowers, these important polyphenols remain in honey, eliminating the notion that artificial honey is just as good...

Unraveling a Mechanism of Honey Antibacterial Action: Polyphenol/H2O2-Induced Oxidative Effect on Bacterial Cell Growth and on DNA Degradation

FoodChemistry, 24 January 2012

Several compounds with antibacterial activities were identified in honey. However, a mechanism by which they lead to bacterial growth inhibition and bacterial death remains still unknown.

We recently found that honeys possess DNA degrading activity mediated by honey hydrogen peroxide and an unknown honey component(s). Here we provide evidence that active honeys (MIC90 of 6.25% to 12.5% v/v) possessed significantly higher levels of phenolics (p<0.02) of higher radical scavenging activities (p<0.005) than honeys of average activity.

Removal of H2O2 by catalase eliminated bacteriostatic activities caused by both phenolics and H2O2 suggesting that the growth inhibition resulted from the coupling chemistry between these compounds. Both phenolics and H2O2 were involved in DNA degradation by honeys. Treatment of plasmid DNA with H2O2 alone did not affect the DNA integrity but H2O2 removal from honey by catalase prevented DNA degradation. Polyphenols extracted from honeys degraded plasmid DNA in the presence of H2O2 and Cu (II) in the Fenton-type reaction. The extent of DNA degradation was inversely related to the polyphenol concentration in this system as well as in honeys. At low content, honey polyphenols exerted pro-oxidant activity damaging to DNA.

In conclusion, honey phenolics with pro-oxidant activities were necessary intermediates that conferred oxidative action of H2O2. Phenolic/H2O2-induced oxidative stress constituted the mechanism of honey bacteriostatic and DNA damaging activities.

Highlights: 

► A coupling chemistry between polyphenols and H2O2 was the mechanism underlying DNA degradation by honey. 
► Honey polyphenols emerged as active intermediates that were necessary to confer oxidative action of hydrogen peroxide. 
► The antioxidant/prooxidant properties of honey polyphenols play a critical role in bacterial DNA degradation. 

Honey Heals Better Than Traditional Burn Treatments

The statistics speak for themselves - honey heals better than SSD treatments used on burn patients - 18 days vs 32 days! In fact, the methodology is completely different. Typical burn unit protocols include scrubbing the wounds, a painful and scarring effect, aimed at keeping bacterial counts low. Honey dressing treatments allow wounds to remain moist, minimizing scarring.  While it may appear unattractive, the antibacterial and anti-inflammatory properties of honey keeps wounds germ-free and speeds healing.

Honey Dressing Versus Silver Sulfadiazene Dressing for Wound Healing in Burn Patients: A Retrospective Study

J Cutan Aesthet Surg, 2011 Sep 4

OBJECTIVE:

The aim was to evaluate the effect of honey dressing and silver sulfadiazene (SSD) dressing on wound healing in burn patients.

MATERIALS AND METHODS: 

We retrospectively reviewed the records of 108 patients (14-68 years of age), with first and second degree burns of less than 50% of the total body surface area admitted to our institution, over a period of 5 years (2004-2008). Fifty-one patients were treated with honey dressings and 57 with SSD. Time elapsed since burn, site, percentage, degree and depth of burns, results of culture sensitivity at various time intervals, duration of healing, formation of post-treatment hypertrophic scar, and/or contracture were recorded and analyzed.

RESULTS:

The average duration of healing was 18.16 and 32.68 days for the honey and SSD group, respectively. Wounds of all patients reporting within 1 h of burns became sterile with the honey dressing in less than 7 days while there was none with SSD. All wounds treated with honey became sterile within 21 days while for SSD-treated wounds, this figure was 36.5%. A complete outcome was seen in 81% of all patients in the "honey group" while in only 37% patients in the "SSD group."

CONCLUSION:

Honey dressings make the wounds sterile in less time, enhance healing, and have a better outcome in terms of hypertropic scars and postburn contractures, as compared to SSD dressings.