Wednesday, August 10, 2011

Algerian Propolis Extract Protects Against Kidney Damage

Another plus from the powerhouse of protection - Propolis!

Polyphenolic fraction of Algerian propolis protects rat kidney against acute oxidative stress induced by doxorubicin
Indian Journal of Nephrology, June 28, 2011

Indian Journal of NephrologyPropolis is a complex resinous hive product, a mixture of wax, sugars and plant exudates collected by bees from plants sources. [1],[2],[3],[4] Its chemical and constituents composition depends on its floral origin, and varies according to climatic and geographical conditions. [5],[6] Flavonoids and phenolic compounds appear to be the principal components responsible for the biological activities. [6] By their antioxidant activity, flavonoids are able to attenuate the development of cancer and inflammatory diseases. [7] Indeed, one of the most exciting recent findings about propolis is its efficacy in cancer prevention and treatment. [8],[9] Propolis inhibits cancer cell growth by increasing the process of apoptosis by a pro-oxidant effect inducing apoptosis in human melanoma cells or by oxygen species production. In addition, propolis can prevent drug side-effects and reduce drug resistance.

Propolis has many anti-oxidant and antiapoptotic properties. Phenolic compounds (flavonoids and phenolic acid derivatives) are the most important pharmacologically active constituents in propolis. However, the constituents of propolis vary widely with climate and location. The major flavonoids detected from the extracts in our study were, pinostrobin chalcone, tectochrysin. We observed some similarities in the qualitative composition between the Algerian and Turkish propolis...

We evaluated the effects of propolis extract on renal oxidative stress induced by doxorubicin throughout an analytical and pharmacological study of the eastern Algerian propolis using thin layer chromatography, ultra-violet-high-performance liquid chromatography) and gas chromatography-mass spectrometry.

Materials and Methods
The pharmacological study was carried out in vivo on Wistar rat pre-treated with propolis extract 100 mg/kg/day for seven days. Doxorubicin at 10 mg/kg of body weight was administered intravenously on Day 7. Serum creatinine concentration, scavenging effect of flavonoids, lipid peroxidation and glutathione concentration were measured. Chemical analysis allowed identification and quantification of the phenolic compounds including pinostrombin chalcone (38.91%), galangin (18.95%), naringenin (14.27%), tectochrysin (25.09%), methoxychrysin (1.14%) and a prenylated coumarin compound suberosin (1.65%). The total flavonoid concentration in the propolis extract was 370 mg (quercetin equivalents QE) /g dry weight (QE/g DWPE)...

Propolis extract (EEP) restored the renal functions and reduced the toxic effect of doxorubicin. These data show a protective effect of Algerian propolis extract (EEP) against doxorubicin-induced oxidative stress. 

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